Imaging-Based Phenomics and Genomics for Diagnosis

Linking genes to disease needs phenotypes that can be measured at scale, and diagnosis needs genomic information that can be read quickly and accurately. We develop imaging-based phenotyping and genome analysis for both.

What we study

  • Imaging-based phenomics. Automated imaging of Xenopus embryos and tissues to score phenotypes in disease models and chemical screens, for example to identify agents that act on the mucociliary epithelium.
  • Microbial diagnostics. Genome-informed probes and assays for fast identification of pathogenic bacteria, including FRET-based peptide nucleic acid probes and quantitative FISH of magnetically captured bacteria for early detection of infection.
  • Microbial genomics. Analysis of the blood microbiome and of bacterial genome evolution and antibiotic resistance, building on our long-term work on Pseudomonas aeruginosa.
  • Molecular measurement methods. Proteomics and transcriptomics methods, such as characterizing the ciliary proteome, that make phenotypes measurable at the molecular level.

Selected publications

Leading studies

  • Kim S#, Hyun H#, Im JK, Lee MS, Koh H, Kang D, Nho SH, Kang JH*, Kwon T*, Kim H*. (2024) Fast and accurate multi-bacterial identification using cleavable and FRET-based peptide nucleic acid probes. , Biosens Bioelectron., 271:116950. PMID:39631211 doi:10.1016/j.bios.2024.116950.
  • Lee MS#, Hyun H#, Park I#, Kim S#, Jang D-H, Kim S, Im J-K, Kim H*, Lee JH*, Kwon T*, and Kang JH* (2022) Quantitative fluorescence in situ hybridization (FISH) of magnetically confined bacteria enables early detection of human bacteremia , Small Methods, e2101239 PMID:35112812 doi:10.1002/smtd.202101239
  • Hyun H#, Lee MS#, Park I#, Ko HS, Yun S, Jang DH, Kim S, Kim H, Kang JH*, Lee JH*, Kwon T** (2021) Analysis of Porcine Model of Fecal-Induced Peritonitis Reveals the Tropism of Blood Microbiome , Front. Cell. Infect. Microbiol., 11:676650 PMID:34527598 doi:10.3389/fcimb.2021.676650
  • Yang HS#, Sim HJ#, Cho H, Bang WY, Kim HE, Kwon TK, Kwon T*, Park TJ* (2020) Alpha-tocopherol exerts protective function against the mucotoxicity of particulate matter in amphibian and human goblet cells , Sci Rep., 10(1):6224 PMID:32277121 doi:10.1038/s41598-020-63085-6
  • Sim HJ#, Yun S#, Kim HE, Kwon KY, Kim GH, Yun S, Kim BG, Myung K, Park TJ*, Kwon T* (2020) Simple Method To Characterize the Ciliary Proteome of Multiciliated Cells, J Proteome Res., 19(1):391-400 PMID:31689115 doi:10.1021/acs.jproteome.9b00589
  • Huse HK#, Kwon T#, Zlosnik JEA, Speert DP, Marcotte EM, Whiteley M (2010) Parallel evolution in Pseudomonas aeruginosa over 39,000 generations in vivo , mBio, 1(4):e00199-10 PMID:20856824 doi:10.1128/mBio.00199-10

Collaborative works

Table of Contents